In this paper, theoretical analysis is made for the influence extent of the rise-and-fall flight on the GT aerial gravity measurement. In order to verify the measurement effect of the rise-and-fall flight, the authors carried out a series of tests on smooth rise-and-fall flight, and made evaluation on the results of aerial gravity smooth rise-and-fall flying measurement performed by GT-2A helicopter and fixed wing helicopter respectively. The results show that the adoption of smooth rise-and-fall flight under the condition of slope not in excess of 3.0° (rate-of-climb about 1/20) is feasible. The coincidence precisions of repeated measurements are exclusively less than 0.7 mGal, thus meeting the requirements of the high-precision measurement.