Abstract: This paper aims to study the coupling and coordination relationships between the urbanization and ecological environment in the areas along the Beijing-Hangzhou Grand Canal from 1992 to 2018. To this end, it builds the remote sensing ecological index (RSEI) based on Landsat data to characterize the quality of the ecological environment and establishes the compounded night light index (CNLI) based on DMSP/OLS and NPP/VIIRS nighttime light (NTL) data to characterize the urbanization level. Meanwhile, it determines the coordinated development pattern between the urbanization and ecological environment in the area by applying a coupling and coordination degree model and its classification principles. The results are as follows. ① The urban development level along the Beijing-Hangzhou Grand Canal is characterized by spatial imbalance. It is high in the southern and northern areas but low in the middle areas. During 1992—2018, the number of cities with a high urbanization level increased year by year. The periods from 1992 to 2002, from 2002 to 2013, and from 2013 to 2018 witnessed the slow development, the accelerated development, and the steady improvement of urbanization, respectively. ② During 1992—2018, the RSEI values were all greater than 0.4, indicating a high-quality ecological environment. The ecological environment in the areas along the canal was relatively stable from 1992 to 2002, improved from 2002 to 2007, and deteriorated from 2007 to 2018 to a certain degree. ③ During 1992—2018, the coupling and coordination degree between urbanization and the ecological environment increased first and then decreased. In terms of the coordinated development type, urbanization lagged behind the ecological environment firstly and then the latter gradually lagged behind the former, indicating that the quality of the ecological environment needs further improving. That is, it is necessary to strengthen the protection of the ecological environment while developing the economy along the Beijing-Hangzhou Grand Canal.