Abstract: Assessing regional habitat quality holds great significance for maintaining regional biodiversity, enhancing human well-being, and achieving regional sustainable development. Based on the land use data of 2000, 2010, and 2020, this study analyzed the spatiotemporal characteristics of the habitat quality in the ecological green integrated demonstration area in the Yangtze River Delta using the InVEST model and the habitat quality index method. Furthermore, this study explored the relationship between regional habitat quality and land use. Key findings are as follows: ① From 2000 to 2020, the study area exhibited moderate habitat quality, with the habitat quality index trending downward and the habitat degradation gradually mitigating. Regarding the districts and counties in this area, Qingpu District of Shanghai, Wujiang District of Suzhou, Jiangsu, and Jiashan County of Jiaxing, Zhejiang (the two districts and one county) showed a downward trend in the habitat quality from 2000 to 2010. In contrast, from 2010 to 2020, Qingpu District and Jiashan County exhibited improved habitat quality, while Wujiang District still maintained a downward trend in the habitat quality; ② From 2000 to 2020, the study area primarily featured moderate habitat, with major land types including cultivated land and grassland. During this period, the water and wetland areas in the north and center, respectively exhibited the highest habitat quality, while the construction land in the study area displayed poor and inferior habitat; ③ There is a strong correlation between the habitat quality and land use structure in the study area. Specifically, areas with more intense changes in land use feature more significant variations in habitat quality. The results of this study will provide a reference for biodiversity conservation and land use management in the study area.