Abstract: Glacier mass balance, a sensitive indicator of climate change, holds significant importance for regional water resource management and glacier hazard prevention. Recent years have witnessed intensified climate warming and rapid glacier retreat in the Tibetan Plateau. However, the variations of glacier mass balance in the western Nyainqentanglha Range remained poorly understood. Given this, this study investigated the variations in glacier elevation and mass balance from 2000 to 2022 in the western Nyainqentanglha Range. These variations were derived from the optical stereo mapping data obtained by the Ziyuan-3 satellite and topographic data obtained by the Shuttle Radar Topography Mission (SRTM) using the geodetic method. The results indicate that the glaciers in the western Nyainqentanglha Range experienced an overall ablation from 2000 to 2022, with variations in elevation and mass balance estimated to be -9.90 ± 0.38 m and -0.38±0.03 m·w.e./a, respectively. Specifically, the north-facing slope exhibited a mass balance change of -0.48±0.12 m·w.e./a while the south-facing slope showed -0.35±0.04 m·w.e./a, indicating greater mass balance loss on the north-facing slope. This study verified the feasibility of high-quality optical stereo mapping satellite data to estimate the interannual mass balance of mountain glaciers. Moreover, it obtained the latest monitored glacier mass balance in the western Nyainqentanglha Range, providing a data basis for water resource management in this region.