Abstract: In recent years, global warming has caused significant glacier retreats in the Qinghai-Tibet Plateau, leading to the rapid expansion of glacial lakes and an increased threat of glacial lake outburst floods (GLOFs). The Yadong River basin, located in Shigatse, Tibet, hosts a large number of glacial lakes. In 1940, a GLOF from Qiongbixiama Co severely damaged Yadong County 40 km downstream, causing house collapse and infrastructure destruction. Therefore, assessing the hazards of potential GLOSs in the Yadong River basin is vital for protecting the safety of people’s lives and property. This study conducted a survey and assessment of potential GLOFs based on Gaofen satellite data and Landsat remote sensing images. It derived basic elements including glaciers, glacial lakes, and moraines through remote sensing image interpretation. The results show that the Yadong River basin develops 28 glaciers and 228 glacial lakes, covering total areas of 34.03 km² and 7.79 km², respectively. The past 30 years have seen slight retreats of glaciers and slight expansions of glacial lakes. Combined with the elements derived from remote sensing images, the analytic hierarchy process and fuzzy comprehensive assessment were employed to preliminarily assess the hazard of regional glacial lakes, delineating the distribution of potentially hazardous glacial lakes. The assessment results reveal 15 potentially hazardous glacial lakes in the Yadong River basin, including five highly hazardous, eight moderately hazardous, and two slightly hazardous glacial lakes. These lakes are primarily distributed in the northeastern and northwestern high mountain areas in the Yadong River basin.