Abstract: As a central city in the Huaihai Economic Zone, Xuzhou has a long way to go in terms of environmental protection. A map of land use change during 2005—2015 in Xuzhou was prepared according to the remote sensing images of this period. Based on this as well as relevant statistic yearbooks, the land use dynamic degree and land use transfer matrix of Xuzhou during 2005—2015 were calculated using the method of GIS spatial statistics. Then relevant correction coefficients were determined according to the specific conditions of the study area using the equivalent factor method, and the spatial-temporal changes in the ecosystem service values in Xuzhou were quantitatively analyzed. Meanwhile, the relationship between the land use change and ecosystem service value change was investigated. The results are as follows. ① The land use types in Xuzhou are dominated by cultivated land. During 2005—2015, the area of the cultivated land, forest land, water areas, and grassland decreased, the unused land slightly increased, and the construction land considerably increased. Meanwhile, different land use types were drastically converted. In detail, a large area of cultivated land was converted into forest land, grassland, and construction land, and the increased area of the construction land was mainly converted from the cultivated land. ② Among the second-order ecosystem services, the hydrological regulation and waste treatment services possessed the highest values, while the raw material production service showed a low value. During the study period, the value of each individual ecosystem service showed a downward trend, which led to a continuous decrease in the overall ecosystem service value of Xuzhou City. Specifically, the values of the second-order ecosystem services decreased by 2.9×10⁹ yuan in total in the ten years. For the first-order ecosystem service types, the values of cultivated land and forest land ecosystem services decreased by 1×10⁹ yuan and 1.43×10⁹ yuan, respectively, the sum of which accounted for more than 80% of the total reduced value of first-order ecosystem services. ③ The sensitivity index values of the ecosystem services to various land use types were all less than 1 in different stages, indicating an inelastic relationship between the ecosystem value coefficients and the ecosystem service values of various land use types during the study period. Therefore, the ecological value coefficients and calculation method used in this paper are reasonable and reliable, and thus the calculation results are credible.